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For his series of experiments, Kaminsky and his colleagues centered on a genetic mutation in a gene known as SKA2. By looking at mind samples from mentally ill and healthy people, the researchers found that in samples from individuals who had died by suicide, levels of SKA2 were reduced significantly. Within this common mutation, they then found in some subjects an epigenetic modification that modified the way the SKA2 gene functioned without changing the gene's underlying DNA sequence. The modification added chemical substances called methyl groupings to the gene. Higher degrees of methylation were within the same research subjects who had killed themselves then. The higher levels of methylation among suicide decedents had been then replicated in two independent brain cohorts.